1.5 KiB
1.5 KiB
Notes
- Methods
- Whole genome sequencing -> PacBio, along with Illumina for error correction in 1% of contig bases
- Development of a new genome assembler, due to the large genome size (x10 human size) -> MARVEL
- Verification of the genome assembly using non-exonic ultraconserved elements (UCEs) and generation of a gene catalogue using mRNA to determine if these coded for conserved eukaryotic genes
- Previously developed molecular toolkit -> germline transgenesis, CRISPR gene mutation, viral transfection… -> Identification of the regeneration cells
- Findings
- Future work
Methods
Whole genome sequencing -> PacBio, along with Illumina for error correction in 1% of contig bases
Development of a new genome assembler, due to the large genome size (x10 human size) -> MARVEL
Verification of the genome assembly using non-exonic ultraconserved elements (UCEs) and generation of a gene catalogue using mRNA to determine if these coded for conserved eukaryotic genes
Previously developed molecular toolkit -> germline transgenesis, CRISPR gene mutation, viral transfection… -> Identification of the regeneration cells
Findings
The median intron size is quite large (12-17 times compared to humans) -> Smaller intron sizes in non-developmental genes, which could facilitate rapid transcription and upregulation
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Pax family of genes:
Presence Absence Pax10 Pax7 Pax3
Pax3 and its cis-regulatory elements seem to be absent due to a deletion, although experiments performed using CRISPR mutate the gene show that Pax7 performs the functions of Pax3 along with the usual Pax7 functions in axolotl
Limb regeneration
Use of published mRNA and miRNA transcripts and the authors' own transcriptional profiling -> identification of 5 upregulated transcripts
| Protein | Function |
| similar to tectorin | Extracellular matrix |
| Ly6 | uPAR surface receptor |
Mapping non-coding RNAs -> 93 pre-miRNA, 42 novel miRNAs
Future work
Model organism to study the evolutionary basis of its regeneration ability