Generate FASTQ files from the simulated repertoire

.gitignore

@@ -1,2 +1 @@
-*.fasta
 *.fastq

generation.sh

@@ -11,14 +11,11 @@ fi
 
 sequences=$1
 number_of_reads=$2
-read_mean_size=350
-read_variance_size=0.0
 data_directory="data/"
-fasta=".fasta"
 fastq=".fastq"
 filename="sequence"
 prefix="curesim_"
 
-Rscript src/repertoire.r "$sequences" &&
+Rscript src/repertoire.r "$sequences" "$number_of_reads" &&
-	java -jar tools/CuReSim.jar -n $((number_of_reads * sequences)) -m "$read_mean_size" -sd "$read_variance_size" -f "$data_directory$filename$fasta" -o "$data_directory$prefix$filename$fastq"
+	java -jar tools/CuReSim.jar -f "$data_directory$filename$fastq" -o "$data_directory$prefix$filename$fastq"
 rm "$data_directory/log.txt"

src/alignment.r

@@ -55,7 +55,6 @@ get_hvr_sequences <- function(sequences, vdj_segments) {
   df <- fetch_vj_sequences(sequences, vdj_segments)
   v_alignment <- parallel::mcmapply(sequences, df$v_seq, FUN = align_sequence)
   j_alignment <- parallel::mcmapply(sequences, df$j_seq, FUN = align_sequence)
-  print(v_alignment)
 }
 
 data <- parse_data(file = "data/curesim_sequence.fastq")

src/repertoire.r

@@ -11,26 +11,32 @@ generate_repertoire <- function(number_of_sequences) {
 }
 
 save_data <- function(data) {
-  Biostrings::writeXStringSet(data$sequence, "data/sequence.fasta")
+  Biostrings::writeXStringSet(data$sequence,
-  Biostrings::writeXStringSet(data$junction, "data/HVR.fasta")
+    "data/sequence.fastq",
+    format = "fastq"
+  )
+  Biostrings::writeXStringSet(data$junction, "data/HVR.fastq", format = "fastq")
 }
 
-process_data <- function(data) {
+process_data <- function(data, reads) {
   dna_sequence <- Biostrings::DNAStringSet(data$sequence)
   data$sequence <- Biostrings::reverseComplement(dna_sequence)
   names(data$sequence) <- paste(rownames(data), data$v_call, data$j_call, " ")
-  return(data)
+  data$junction <- Biostrings::DNAStringSet(data$junction)
+  names(data$junction) <- rownames(data)
+  amplified_data <- data[rep(seq_len(nrow(data)), reads), ]
+  return(amplified_data)
 }
 
 parse_cli_arguments <- function() {
   args <- commandArgs(trailingOnly = TRUE)
-  if (length(args) != 1) {
+  if (length(args) != 2) {
-    stop("usage: repertoire.r <number of sequences>")
+    stop("usage: repertoire.r <number of sequences> <number of reads>")
   }
-  return(args[1])
+  return(args)
 }
 
-argument <- parse_cli_arguments()
+args <- parse_cli_arguments()
-repertoire <- generate_repertoire(number_of_sequences = as.integer(argument))
+repertoire <- generate_repertoire(number_of_sequences = as.integer(args[1]))
-data <- process_data(data = repertoire)
+data <- process_data(data = repertoire, reads = args[2])
 save_data(data)