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* locigenesis
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locigenesis is a tool that generates a human T-cell receptor (TCR), runs it through a sequence reader simulation tool and extracts CDR3.
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The goal of this project is to generate both HVR sequences with and without sequencing errors, in order to create datasets for a Machine Learning algorithm.
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** Technologies
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- [[https://github.com/GreiffLab/immuneSIM/][immuneSIM]]: in silico generation of human and mouse BCR and TCR repertoires
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- [[http://www.pegase-biosciences.com/curesim-a-customized-read-simulator/][CuReSim]]: read simulator that mimics Ion Torrent sequencing
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locigenesis is a tool that generates an immune repertoire and runs it through a sequence reader simulation tool, to generate sequencing errors.
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** Installation
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12
shell.nix
12
shell.nix
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@ -21,10 +21,16 @@ let
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installPhase = ''
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mkdir -pv $out/share/java $out/bin
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cp -r ${src} $out/share/java/${name}
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makeWrapper ${jre}/bin/java $out/bin/CuReSim --add-flags "-jar $out/share/java/${name}/${name}.jar"
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makeWrapper ${pkgs.jdk}/bin/java $out/bin/CuReSim --add-flags "-jar $out/share/java/${name}/${name}.jar"
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'';
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};
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in mkShell {
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buildInputs =
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[ R rPackages.immuneSIM rPackages.Biostrings rPackages.stringr CuReSim ];
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buildInputs = [
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R
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rPackages.immuneSIM
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rPackages.Biostrings
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rPackages.stringr
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jdk
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CuReSim
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];
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}
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