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* locigenesis
locigenesis is a tool that generates a human T-cell receptor (TCR), runs it through a sequence reader simulation tool and extracts CDR3.
The goal of this project is to generate both HVR sequences with and without sequencing errors, in order to create datasets for a Machine Learning algorithm.
** Technologies
- [[https://github.com/GreiffLab/immuneSIM/][immuneSIM]]: in silico generation of human and mouse BCR and TCR repertoires
- [[http://www.pegase-biosciences.com/curesim-a-customized-read-simulator/][CuReSim]]: read simulator that mimics Ion Torrent sequencing
locigenesis is a tool that generates an immune repertoire and runs it through a sequence reader simulation tool, to generate sequencing errors.
** Installation

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@ -21,10 +21,16 @@ let
installPhase = ''
mkdir -pv $out/share/java $out/bin
cp -r ${src} $out/share/java/${name}
makeWrapper ${jre}/bin/java $out/bin/CuReSim --add-flags "-jar $out/share/java/${name}/${name}.jar"
makeWrapper ${pkgs.jdk}/bin/java $out/bin/CuReSim --add-flags "-jar $out/share/java/${name}/${name}.jar"
'';
};
in mkShell {
buildInputs =
[ R rPackages.immuneSIM rPackages.Biostrings rPackages.stringr CuReSim ];
buildInputs = [
R
rPackages.immuneSIM
rPackages.Biostrings
rPackages.stringr
jdk
CuReSim
];
}