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2 changed files with 10 additions and 11 deletions

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* locigenesis * locigenesis
locigenesis is a tool that generates a human T-cell receptor (TCR), runs it through a sequence reader simulation tool and extracts CDR3. locigenesis is a tool that generates an immune repertoire and runs it through a sequence reader simulation tool, to generate sequencing errors.
The goal of this project is to generate both HVR sequences with and without sequencing errors, in order to create datasets for a Machine Learning algorithm.
** Technologies
- [[https://github.com/GreiffLab/immuneSIM/][immuneSIM]]: in silico generation of human and mouse BCR and TCR repertoires
- [[http://www.pegase-biosciences.com/curesim-a-customized-read-simulator/][CuReSim]]: read simulator that mimics Ion Torrent sequencing
** Installation ** Installation

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@ -21,10 +21,16 @@ let
installPhase = '' installPhase = ''
mkdir -pv $out/share/java $out/bin mkdir -pv $out/share/java $out/bin
cp -r ${src} $out/share/java/${name} cp -r ${src} $out/share/java/${name}
makeWrapper ${jre}/bin/java $out/bin/CuReSim --add-flags "-jar $out/share/java/${name}/${name}.jar" makeWrapper ${pkgs.jdk}/bin/java $out/bin/CuReSim --add-flags "-jar $out/share/java/${name}/${name}.jar"
''; '';
}; };
in mkShell { in mkShell {
buildInputs = buildInputs = [
[ R rPackages.immuneSIM rPackages.Biostrings rPackages.stringr CuReSim ]; R
rPackages.immuneSIM
rPackages.Biostrings
rPackages.stringr
jdk
CuReSim
];
} }