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Author SHA1 Message Date
coolneng 18ffbf9a75
Add v_call and j_call to sequence ID 2021-04-21 18:51:08 +02:00
1 changed files with 1 additions and 7 deletions

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@ -10,19 +10,13 @@ generate_repertoire <- function(number_of_sequences) {
)) ))
} }
save_data <- function(data, reads) {
vdj_sequences <- data[-1]
amplified_vdj <- vdj_sequences[rep(seq_len(nrow(vdj_sequences)), reads), ]
Biostrings::writeXStringSet(data$sequence, "data/sequence.fasta")
}
process_data <- function(repertoire, reads) { process_data <- function(repertoire, reads) {
columns <- c("sequence", "v_call", "j_call") columns <- c("sequence", "v_call", "j_call")
data <- repertoire[, columns] data <- repertoire[, columns]
dna_sequence <- Biostrings::DNAStringSet(data$sequence) dna_sequence <- Biostrings::DNAStringSet(data$sequence)
data$sequence <- Biostrings::reverseComplement(dna_sequence) data$sequence <- Biostrings::reverseComplement(dna_sequence)
names(data$sequence) <- paste(rownames(data), data$v_call, data$j_call) names(data$sequence) <- paste(rownames(data), data$v_call, data$j_call)
save_data(data, reads) Biostrings::writeXStringSet(data$sequence, "data/sequence.fasta")
} }
parse_cli_arguments <- function() { parse_cli_arguments <- function() {