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576597cb04
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coolneng | 576597cb04 |
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@ -19,6 +19,5 @@ fastq=".fastq"
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filename="sequence"
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filename="sequence"
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prefix="curesim_"
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prefix="curesim_"
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Rscript src/repertoire.r "$sequences" "$sequencing_runs"
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Rscript src/repertoire.r "$sequences" && java -jar tools/CuReSim.jar -n "$sequencing_runs" -m "$read_mean_size" -sd "$read_variance_size" -f "$data_directory$filename$fasta" -o "$data_directory$prefix$filename$fastq"
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java -jar tools/CuReSim.jar -n "$sequencing_runs" -m "$read_mean_size" -sd "$read_variance_size" -f "$data_directory$filename$fasta" -o "$data_directory$prefix$filename$fastq"
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rm "$data_directory/log.txt"
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rm "$data_directory/log.txt"
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@ -22,7 +22,8 @@ process_data <- function(repertoire) {
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"d_sequence_alignment", "j_sequence_alignment"
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"d_sequence_alignment", "j_sequence_alignment"
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)
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)
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data <- repertoire[, columns]
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data <- repertoire[, columns]
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data$sequence <- Biostrings::reverseComplement(data$sequence)
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dna_sequence <- Biostrings::DNAStringSet(data$sequence)
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data$sequence <- Biostrings::reverseComplement(dna_sequence)
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save_data(data)
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save_data(data)
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}
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}
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@ -34,6 +35,6 @@ parse_cli_arguments <- function() {
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return(args[1])
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return(args[1])
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}
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}
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arguments <- parse_cli_arguments(commandArgs(trailing))
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args <- parse_cli_arguments()
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repertoire <- generate_repertoire(number_of_sequences = arguments[1])
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repertoire <- generate_repertoire(number_of_sequences = as.integer(args[1]))
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process_data(repertoire)
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process_data(repertoire)
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