Consider the read start in the Cys location
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@ -110,8 +110,9 @@ get_cys_coordinates <- function(alignment) {
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insertion <- unlist(Biostrings::insertion(alignment))
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deletion <- unlist(Biostrings::deletion(alignment))
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delta_coordinates <- handle_indels(insertion, deletion, cys, alignment)
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cys_start <- cys$start + delta_coordinates$start
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cys_end <- cys$end + delta_coordinates$end
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read_start <- unlist(start(Biostrings::Views(alignment)))
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cys_start <- cys$start + delta_coordinates$start + read_start - 1
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cys_end <- cys$end + delta_coordinates$end + read_start
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return(list("start" = cys_start, "end" = cys_end))
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}
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