locigenesis/src/repertoire.r

library(immuneSIM)
library(Biostrings)

generate_repertoire <- function(number_of_sequences) {
  return(immuneSIM(
    number_of_seqs = number_of_sequences,
    species = "hs",
    receptor = "tr",
    chain = "b"
  ))
}

amplify_rows <- function(data, column, factor) {
  if (column == "sequence") {
    dna_string <- Biostrings::DNAStringSet(data)
    reverse_complement <- Biostrings::reverseComplement(dna_string)
    return(rep(reverse_complement, factor))
  }
  return(rep(data, factor))
}

save_data <- function(data) {
  Biostrings::writeXStringSet(data$sequence, "data/sequence.fasta")
  vdj_sequences <- data[-1]
  write.csv(vdj_sequences, "data/vdj_alignment.csv", row.names = FALSE)
}

process_data <- function(repertoire, sequencing_runs) {
  columns <- c(
    "sequence", "v_sequence_alignment",
    "d_sequence_alignment", "j_sequence_alignment"
  )
  data <- repertoire[, columns]
  amplified_data <- mapply(data, names(data),
    sequencing_runs,
    FUN = amplify_rows
  )
  save_data(amplified_data)
}

parse_cli_arguments <- function(args) {
  if (length(args) != 2) {
    stop("usage: repertoire.r <number of sequences> <sequencing_runs>")
  }
  return(c(args[1], args[2]))
}

args <- commandArgs(trailingOnly = TRUE)
arguments <- parse_cli_arguments(args)
number_of_sequences <- as.integer(arguments[1])
sequencing_runs <- as.integer(arguments[2])
repertoire <- generate_repertoire(number_of_sequences)
process_data(repertoire, sequencing_runs)