From e39593bbc9debd0f1f9c39ec6d2bd42c0454d64e Mon Sep 17 00:00:00 2001 From: coolneng Date: Tue, 13 Jul 2021 10:57:42 +0200 Subject: [PATCH] Add DNA sequencing to the slides --- Presentation.org | 7 +++++++ Presentation.pdf | Bin 237851 -> 238992 bytes 2 files changed, 7 insertions(+) diff --git a/Presentation.org b/Presentation.org index 66991c6..1a9b201 100644 --- a/Presentation.org +++ b/Presentation.org @@ -25,6 +25,13 @@ | Ion Torrent | 1 | - Dificultad para detectar los errores en regiones con alta diversidad (e.g. repertorios inmunológicos) +* Secuenciación del ADN + +- La secuenciación de ADN es el proceso mediante el cual se determina el orden de los nucleótidos en una secuencia de ADN +- *Primera generación*: secuenciación de Sanger +- *Segunda generación*: /NGS/ +- *Tercera generación*: /TGS/ + * Errores de secuenciación Hay distintos tipos de errores de secuenciación del ADN: diff --git a/Presentation.pdf b/Presentation.pdf index a5c7afacca8a02f3ee3396d5aad76a964675c069..ec422e9471cdaeed042084acd355aaac172ca917 100644 GIT binary patch delta 20301 zcmZU)V{~Of*EO1)6DJ+pwrzHtj@_|s>%{4p9osfKwr$%sI=uZn_x;Ab@A!VKjZroB zk6Jb7Ld~7ghZr}GSUU)x7zw}yak8@t3c@+NIGGyS!g;J+YK+^hGb49A)4c$V+*9m@ zXYz#ca$C^5Fa%cOlT?sKzlhpol~@lsg%j5>*Kose;t3NP_!`g)ve9XGh*Sg6!7a#Z z2)pR~m;-)_GF6E@9$sMuO~>+U2)fs#Iii?Eq(OXnsw$N4I;8Uu?n_I8_R%57K8{7A z&-|Q+xDLK~a*|_P3P|JGh-4$^*|^)Al1=|~z=OYYxhbC253E#}lf7Whnp!$B*uS-j zNyqOzw9uQc@+NXS`=C;V!3D&xMVH@}MmLRV+4h#}el$Y3Y;_xO>;lrG=P2|KY_HTAib}d2~ z-Z~oGan>T~?2~<#SdCr()i@4lscJEcLFh`SUl$28-IMIBt*H`TmbR&uCN{;tjPtnZeS(Pw#llvm5cW9cZRq^` zE9dKRGSf7yQ==pV!uTEBJS7T2aQMU$20Fn-yXjD)vyWC*O1qi8U56ng+rJzNTqaL` zE;oTLe@)=eRs za4JrW;ax`iEuP76m!mJbnyupzW8f_(e(;YT^mPM_&yFP)72qc&rR}7RPVHAU;Q87Q z%!vKY4bIfg&Aa+c$90hIjbHeQ@r?0023VlZB#oJ?4m<}u#q0%~qDAll{0oSgDisi(#F}V* zL>>IasD9o3_6>^kt2t%s4V>^l5J`!A0Y?Fi+N}#CbUxD^L9>(a@DWes38VcFERwVS z3yZN;lTpruKjHhu%z;u%zxpo)oTzW96~Vsp9LQmWNww-e8D7Gid?*@E%N5`M@YTOO z^mxyIZPM^fk>dEc1==I+DY@XwFYhTO7ivhv6}-PUqWp9OImXr`XpFiif*^iZC(kXO z(zdpEaGtAEi6#HIS=1Q)7^IWP`R=qPJ#CohVqR4^@3)-MNiGJOPd~nr&*@?um}G#1 zfQ^x&Z_aO^{=L*^Q6>^Oc+&u_sU?gB*7CE50`M1O#|*L&H~%ak3DQaIV&sf)#y$f1 z0Hgn!;r0ue#$F;UP zfVEvgJtEtpVG49d)&ec|S5GY3PicZ<(h^{@*%@|I33=Lxfy?)iB@i2|KblqHZdWSW zD{qO!x8XeiZUh+~5}}1;ldM~!7_U-XzFoA&_h#1HeM?*LXTHUx_8 z@W>eq zK+W$>6^-8s=`QO+hEoguM@o-ESf&hDEX8fcq&(Sh(qfjM{-ASLLu-NSA>83hAMzKe zeBxmKB>iMMHm0GpKv{Rd$cm)%CwC6bsB5}c3_A?bG53BIhqyA0HzjI~Y2v?_CnQlo51m^Qm z&%EFJB1UFzD0T4G8W-8`5SX)%C!)s^xzFKOZTM=>d*YJvuY^SOJ#vgc^yi#DnuYQx zt6Y|j(oN*=_+*IO`Sr^%r$JiuojTp^4wnWyU?m3ISxK5VDXw{nH+X*s{{!=^jX7abiVX_^sdpnc0( z2JQ>D7+>sdVFAc70&sZyT+pVpJZ*Oyc@TL(ln6nlq2TnbSteWV|3sx54)xLbQ7obl zvIK!dJ)^L`|3;<0Nu|VsVa5Bk4ZjDkit4RA(HZ1l3r`Z?2%GBEc`6t*o3aG$!77P1 zp{V7A9gzBHY1*0={{T&SvnK6jVGp5L*9Y_ESIRNF=M^b#3&)Yt2gaUPf$+^CuPpFd_DbeYj~q5x z=p@jvp%`j)L7gM-Rw=qWJ5e3aCBfP>HnRVBxkO|~wy_ERK_x0J-?O^|3h1?{ROOB& zNkRB#jm$xO_fOvd<6-)Q!|3tOVi=#YeV5>yq)OF^QpSMD@GVw{eCUrRdE;`>1*8r_ z)H;15WJXa$dsl>z_}J4D+(7Wvz?P(1NqYE%6XQT7Uc6fNStwaD%weC7aeWj#j}U{# z0-{mRPGdut_Mhh>+nw}FD_HY-&0|N*($;lXK#?h)d5+siFgonFzpg1aO&}ul@Bn&UQ6yPCwsU;t}#`bUypEISyZ}jiH zR5EA#B(H?OxB(sEH=YB@J2LaZ0!6?{4k{reKq^E&vkT@m_UR#d?6yUHZ#ot)QeP;M z9wgZHeUFmw4LLe@*!-57*QtaK^l1^sYC$(+{QmJsN_l&Fh1q@Sv;NnLPvpKu?DL88 zT8Cll!o~cBSWNK9Pq{)>u@BF!u=-jis}v)FbXl9<+^ zzQ8CE$z>B|O(}e#z|};KQbxcwpqwTvp~=_-ds7iYnx##!yx^NS23{~-kY_n6Il@NZlB}$?!1$zjx@>+z_d9d`iBj~&YfHt&@bQ6;Y;HI=x z!iB7+)1L;rGX(j{je2hQ%QMr^YsX5M4V$bHNwZ@L%%t7d|YQ_W?hx+`Lo-R(1kK7lp&TRVQ}{KV4WT%f z7A+dJtcpiUD(tpw6J~RnGlZz0)}Ju@);C3YGTWT3<5DOi^ljkLX2d0Cu&HNpa1Za# zI5YOoSS6JOGF2~0ieL$x406rRW=(66wdu(kd)W0Ff)m5wvy*$~0aWnPaY#ECkERA;nJYK$L^jSHd+W?uaEChOEKzDhah^Hq zJJ>n2JEcZgeU;^p-|@l?ml`wXP-ZF7GVuEORa*o`ObBBJ=b0Q#_(1#_V)IaV9+^+gi2cbi~1v1F1M7c6+AI| z5mO`USb?;}Z%@}GV09$E`b3u*L$WE{=M;*~a6W4pGh10|+0mez^*DvLQ_EN{k$f_E zam|G=M?mQcP^aHRDQ%~iymP;@-oQyi;=*9-ibJ)hNnX{%Y{f$$oC-HI8w0agT)uMTWGWwnUg~+ z#gE6`zTKAT2|5tb)vgDOX~Zf_m#;dL)!{ZE5W+<_uavb;aVq#c(&TVG-PbPe;NOSGD#poP*hCB+}fq~_Sb*X5TbyiqSlZ3 z^=PkqLX5yr;X+R6rn~=B98W$>CzFn72SEh2>PR8X)Z6$P#P_Vggkj<-A9v%*IxadSMp$BqIF@#aM24)Ef}p>EK~ zaYw3;Q>NCkoLqe%j35_GnG(sYy{qendJOS|m&i z-xrjB)!QJXwwH;xLd2g|2p(0n0sCKI9lr5-m^T=_XMN?`7sYq1X zaP+l^li6#=D41goE=4hiC}R*I;K@aF2ipl{a-M8EI9=c5hE3IM$Zq{ea#Rn$;upX8 zcZ({@eWtj!kSZ3D%d{2thf_PQgo zBi6LtfE21nvYCzIeAB%Tk+@QMU{U>!_)N@tE2{WYJKvQ%({SLMu-{v?vnjYHCY#}0 z{67RL|B@UmzQ4S^>dR~E@KQbv3dq(s9_;$76} z=!&VreFx8-K-9U}IZhCx7{k?O2lrzXDg<{L|3!f;eEo%u#D@SN_%ejt({hhTa)*R$ zAqoo>g`S%HwjB(8Ty-V4GmTBq2v$N0t3)5zZ|&UCY>PMS&Onj1{L#THN!ysj*M5HM zrkei#xDDfuAjhQNE%O}Kv+Y($Wjt5CWC1afsC0#txiww%{sJ}JeZz8@mkbW%2Jx`7 z|8FLcRoGxg?t0dEK_Z`s+Mc5glYuPbD(_SinDKQu1ONQW+po*wHdG3@s%IenE|pm) z5NqExEwV|Y+9OoTPXjoK&s=*~_9+n1ht+MfQr)gbMA+KC+j#JP%*3IpixSD)5o$S; zcg2_2*q@Dg%>`AFD%|;Wt9ZNmy2T373(?1#!Sd=76jg8yI()sAbIhp4T+fD%{ zCR9_DBv!GKD8{guyCOLx9JYey{5y&44>QG`TBb1g&IBxDgQ`01b+hp9@eV6JGB%bU zpUZ(*08}^rt(eixFB0S6oV{PAcLVA;l7iR4n)D7#bcYxRRc@EGBJeWy2Ei60l`B8I z!*p3o8$do2*Xvnhswz4PrYj4+JjKj0Ed;Eu94@Jw2J7h!A{k{R$|35xav+{F;N58wO3`e&Pia7k#G?qp{@>-LQdjAnxP7#<(97PMovo&&R$|B(_&=;`i5;G(hg!V&f2LnI(ZLR9R2W;3*bDVqe7pJYS zqzBCMDEk^&--PS6N2j=(^}(R!QBEO%AcESr5I#i zd*o@qRJ#s;3+Q@dDs#$|`MrcMqzDCpW&zLvIdr`=2^vDi##?}EiA>aUT2$Z$5h?k0 zyG1dF+v=o@%x{Hc^Xb)awW&;egvA11Px%SWowOp_#HG?9%SX;OjTG{Q(+%NcDA{vJ z?36>nL*_%Xr@xQnOusl7m0)^AvT~zVl!&BHy)a~RLMlp#md*5pKE?kf16%40 zdkauKEfu57Iex%j)&*++cjJL0(H}qoum3WUMRX2Sd3MYz`HaenVNsPBz$j`f#7 z&mHbxQQ&ICN0C5^q(I+NL&=zz{{5e7L|cepP*b451r) zf7FHW1R1DA=JWML$RGA$}{%%=SfG3sZV^EmTb``&ogGSG?%0zE1P zN;5KNXb=D`is6JxyIJ z-kfc&R)%Dvo=9R$%UKf>e>7*!teTr(3WPy!;xuSj$8C{IQ)x^%CAekGRi%kb2lv~P zH&uzKYX{=!C9U0z&=4XT+m_h*fhOQSaXuU5%6!UPS0-3)$CZJI?bjU{ar_XkC+u95 zoKD9aeBrz(>IBoRK8^!IU%q%-St&DCwj0Vv~uDG{PXgR)a%n_MH3t6@s-TU~0LwN=9T zU<>vKWr8tPXGq9eR%gR(iLj5SE2R?}!tDuuMs8O{h zfeM|$N{^ZtZi{$z%Ch=m3)JfrbQdc2S<4Z`PA&n!o(!XKLAuvVEW>*&Ll~cT1JSak zoIkQc$zICX`SsIBnPN0p+EFUCfIvWb{k|r?`N=e7JK%bDO03O_s?@f=sQ+S#^~Jck zkM;)$W{=HFg_ps)y|+4kUs!v;yw18j0Jnqo>OAJ?D(3Q~i<31xHF8Jdg$G=hK=p01u=FnV75u*>vIL znBI+=CDwnNN@IfJSv~O7`@8(k0Nfcwo_`+Yi=HHT-X%@0yb?&wIq&V2ZjE^HaY^E{ znYJ~cHcY594ob?ip;;tTiO-bHA`#iw^3R&hok)enbaLDF-; z6;KOYi+vY(!cK=&3JV4h9h~bw$8{+^$Uqd(v|Rx+^3XMZNUW|JMl*PZ6bkC@G;P>s zRq_Kk3^$FkaU4ll$48%!JW?dYiFvGf7tVC>U>*OOq2X>jUi%RJ1?$RInrBD*RBb~m zA}4afqgabazP-D32{FB2)BN?5nWl$_&}zH=w2>FRP{6@JjmIp7v_ z%7Q~FKs0{D!gS=;m=b^a3~s#ZWs{2aY{29xlsy@1dl`Ekjd$_8(+B-q75lU0D=U{V z)=&^VnLFUDt&Zz{!ELDN$tqAlKPT$){Baau{Py*e(@K0bqAy1*I3LV1KO#tZ2K@VPCWu+u>8uIR z_R=`gIXUtor6tAqCa?^+JgTiV18l=d1zRY8e_$9%@Ng7d^WI+cnok!mG@K)~e((Ef z3_9PleeDXXY3FO10z^~)Smi_x;@DQc!HvN zU&O&heOxl}firPp-w;lrxKI;-BuFAOSAg6vYL(^%o`0s5@dL30@%$I&kAi) zf%--Xo`*(pwh`i7ndP(+1s>vq8C-_FBoA56`D?F!rdQJbWR~XRBmnk9sdDh_a5J|R zi`b(AAs7}prvXZZ0$TLPz-B!rOE_I4jhc%xoG4V;3v1|4DkAqAM{|bl)xSvBFmr8j4LnR7~-M8a}qE=>SA%{zdyU@pUoob91)kyNX%KY5r8tgzGcE@%9PF#Y`3%$UnSqG^Xgv zfY7OGP{bov8&OPWUSZXC2PXG+4a%Nv-j~)bbRAE0(IynX_q-w}O(3QyMEGr5NMSW9 z4T7|~Y!bIMRvnYJRt_wu){D5^JKZHWS_qU!fh{AR9Wxb|QGz@qg$O5wnP6j-odY!K z#xYwH{`J61*bd{kSsA8N+BgoxxE-hvEkY-WR19<)t<}EjRu1L3fgBvKh3d-lm`7d4 z-3Bg`a~VwkRsP4X>ig(>$gO_Xz-f_7xc05fzBi;Ri4N!gcm)l>!TevhuyS(zFWf8k zMg%bkV`&GWq1i6EEO!fjMQm1alfuQJ*FmDMrBusf7tX5JM?pZMK5nYIQsK+!d{Y zQ6DzhWFcCT*66q6KPQTM=ov>ADSbpAmCnkp`h;y7UV&eqSivtk`Zc+YslHyv$KjWO z+>%A!PL4#ai+pS0{-QbKEj;+iPR!G|rrkGm`4bz(CCru`Uvz>kvvi z@_qWBMPm7{YK4u9HN_qqND7J*ookfNZIO^{kE^VojqLDs^&1t33{h}*17)%#>b9xW zV;w2A9EN)-$*Ng;H3l(C>y*Wh)eo{DQ-xo>Jx?K>>moGw5_z1 z>-wp%ZNPPQWIC^7VJvF;__>j1uC_ls_&m65m$hMJM3V&bB#%ngXK>(b4*Y3R3|Kx<_vH!K@X$FY62TaAxm61bxQ@1KMGx$Zz6t&O>u6c zadR|=Ip#s6>t~cl%wt>;l_DtIC~YNE8824y{-ModA;6h^94~2g)}{J@yIOr&OxyJZ zHn7+w`af&V{%_4GQs8)iC@Gw{Ks*2kM~ezBFrM(g5~BYOFr?hL0O4Cid4LcU08ZBb z&bQSA(&2Ej{eMZ1oT;6;iv@+ax`_ zBzg^+rtCN6WvO&J^^ZCBAM?c@@s6xj`=h; ztCsD~?G_~TJ{S@KFBr#C&uJ4CYoBAJF@qzzn}Ng<^}jFqw=kj}c>I%+DY04Hzh8Ge zgGZ=Eq0Z|<8YG2YA0mTbeitIs%1;)dmGx~w8~VPvu3YB?!A>s2n3uPWM1t9| zQZ(kqw-q#nViec6dtZ^_BUFu1%0#j`P<6WlBrIaJZwwbA_zcx+_D)EZ2T`=b2vjwm zU=_qgPYg{)s-1JjyF!tld31Wn-tl$GyqvAZm@&o=$ND_yk3d;5|Cs3?EYnRM||RI5+NAOA3K z{fxYud*POYH0IPh%T<)5A2K(&_%2f(Mq@f4JL%GyW~7G~ zdq+H}9hd?)T;Q0xd#X(m@{WEtYcLN2 z77|y8BAP2H*XI~phzc1OsbIPwDax;#hq`V8d(u)E{Y*tsJ&PD==ohUIgi14OLQC4f zi;i7KMXM`CZW#8U;l>H!z*GCNdmX=!U-+$^NsOOO*3y7F7^3zMvW(Qhh!QN72%Plh zNqi%zg7{)sB1l8+1V({Is@Eebe}GI*HOoV*LUQ+0u|UB74REWjk3Obai}xKSXv?t+ z^g~Lqo7tNLuc>TcU3erjX@`IWC0^^~x>nbzhtXtR(e}QWG@iyqOMfJw!8TA}$>LcG zibYJSJ4xm{zj7VSFMcMBkg!uXuwX?w)|pv|CQef_y*}y!unu z6&l(M8N>>2P*of3#bg|nRx?35Mm#5k@mnSsd7J5!0A<`P|A{xfS4p*8q_cY=SG_0M$w*}1UL+H#OzBbInqSV);9MDq|g5!Cij|HN;H zhwr!*dP;YHD3CJyD7nFc-U&LW$$25tQMQr$C7SG~+A)6?6%R$>{(LQn8z}_^GGs8^ zzaFZ`+ne9Lf+0P3XTxSrF{I6VntT8%%;NR($mSy$R9iWj!K6BxdK_p`2?)z{ACjLv z$iFi3L?Nia1;7MF)Tyoyp-)Gle_m<-#6yQ7QTv|vUDzzdWoLLr8H)1KAKmn~4EPU7 zt@+WJ!oXU-&La$uF7t0Eu3HhHK5tX)1UNIW#FRaDONeKQ8szMKTa90l6%ip8q>Cc* znApwCV3OQEZ=O-}CX~zHP-99U!r`fuYvuZ2@p>H7N9?u}!Rm-R;ek7H;M@rQelskL z!uIf#ftjpoaTGnUR=?;U&r1Q~ zvmh~oa`A$)-5DuKht%2VwWEdR{}kZc~mwhg)20vs5w! zS6oKes73P6xFZt#@JVeTGFt8hI!{B%)G|3^wqrEhWB@)UQzl!Q6~rB4QXKxCJ@P=% zGp$`2CcF3}Ok4=3_%DItkrkxYrv9vkey}LsO*fIc;CyMlQxU~AQX+>xdOcd4-bI|; z(_-$a+mHtiXk3RM)S{n_b^7he8DcEVc1UmJs`)OmHZD{u?PFn(~CV2c%^Q! zU4HGcScKjo03A~A3VY}er31MC8N?tC*bdBKo^ZmRBC)t5b)1<~2pI3!LH-O2C058$ zst$U(xswv;MDX8R3|lL8W6!@K*jh)8t$;`y@<+fQ@*qBNoni^|0^+DGXj9c*t$2|L z=Y(AYNOKHM_v5myX$hNQ8k|Bt`Eg=#A)pI-a09Cp3Spc`#EoCi<>c^*Y@x)8e!`nHIG*|(5{0tzoh;=@Ttz-s2>8xu$}EYH`0~|go^~zU z&wDu^K4P;no-whK+TFgXIsODVnf5olKi{8+|O0SxA;=yOGNLM<3!GuV6C_> zAN2#`*kT5^62pptW;R1^l}TGxcH#)N0CJBWfU6VMB8I32RRs}9#RFi2Wry3w-;2uJ z+T(N%{QK&#%sji@A)-%C0=C@!LoftnHCiDRtAq1{7PYaLTmt%JjXqAq1L^HFZ$<>x zgq>P6RlCXnB3jY7>37-=NlrcXc=?H#{^Ts<3$Fk38o)%ts8%GJ2XVSoxcCKz6k>8D z5CfA`)q;^7=fS_!*~(uqUzH=!;K`Clzal3ML;rQ4TH$Moh@5bp4<&;F}%H(oRY8GM)0SaV$J?0f)xcDCY|o(DiN(lYcSym8-rT_hJn3XFst#W zZfWXoBR~_NZxydISOjm)`zP#qL~adGmO$l!jAw&k!pOf z*nnH9J{|2ptJ)w{l&+yfxIPPN)-D)5T{xo?DRHWbln5JAHhXM>0*GeCs^s@y_k-}N zoEJm+=dZ5?@d?hc2z$5DW9gZ4@czC4TD9pG>?$-rMBiUJ_Jw>`yHC9C;@`R+Yb)uz zU^~sK*p$8 z9#!+n`liqIP?GfU-m&-u==*TJ{8@hW^Jlru}_lS;Tf_ZSJrY)IKj zHrd4j-jemQr4;)Ytc{;`zkz?2)Uax`f5TKe(^UIetex&bcmC72^9>>5-{fpyNe7)% z0r;M|o~{Jhx00`@G|qLV)-Ho|e|*xeAJl$H&8(j|aCfxhuDhu&Mum2$*iJx`)T(`3 zk%_$MwX#CC3mwBI8s$@tY((By<)7I52@{u|a9)?avVAgA^pt@$lIB+HiFNW0ZzQfx zo}1N6_{-9UDG(X7JiE`P1J*kob44%EHoQ8U1-W)_(b$2X=akpc?1gvHs@fU4| z7GECvex6}?hKPEbJBa9heSF;t+UW74GlszDaOcxv3V~G09(Y zVjGpk0K)-=+g7V-G;|xg=%4R(Y07@kLF;4*et86Ku2Cdl+21`kL1XO~0v4 z#fC{dA$UEp7u`UjBVQd60oRp3becq_RWP}3CdOMa zSTsvp_ClrK%D%Ebb6sx5Kf{`J#*eqo;Yg||MU3yFa3vOiUIRJLUeW|NH1n>IP*7});mF1-^Ln2urVV*vPT@tL1 zcm4n!gshmTGz+k9>725(zw+L+q$55crpY~WuA0R>V!rE7m?5&G;*)AlF5>=@sR~k@ z6ZI$yZ6YRxF3W~Y-8+Q%#^FXPPs~gnC{=-M)eOX_5OX*=SP*6tH~^$SA9fHEMchS; z-R@}i8%$~xQ;kc8F(zZFDR{U`IvxYr#2P@PSC-E)utv^Ud^o>_EKu-r_-1D69V{-= z3VzH=_O%c3t~ttvWUl9Z1|bRs-Jc*XWr$et+w^T9KePtrnaKBqvRON69~?ls7^=Sz?@%?tI2X$Kp!8JA_^`3C@XZ2k z3h0IKeP?*PB#a*wi>Do>rH( z^_VP#4}N=GV|9{GY2nfeOLfd`Hjuq^6a9v$MmwrHH*wbJP#hauDy%Yif=IrAJBirP zubkUr%h{2?2X!#7OxJ_;*Nba;YjNn&`#F+ujq4I^uzGiCudLA?dMkr+$-y0Dsr5wD zp{mZy(g|IT3yF9IxqU+9M?2>&NQAZHcmT_puBjueCo_hHnO?^U9SZpy=LaJEq*4n^ zsZ*6U)>A(+R8#vn!?tUR?ARkZYq$NxcVla1aJ# zJ)(l%Oqky2S=d_edB$o@_COe9VCTiS>h_fvBgr(9y zjc?EMV6naOGGWX&JYd_@D zs@LA|g4^3;O#1ER%~ah;1SC(~hLnep*85xX5%s9_Ai@z2d~|*4&VFLB_D*|ROD8QR z38(_C5w(UNyMYQ?GCwc^1e)w_A`FN5I|3ls=jT^J3Hk+&3TW77SPOM#fL$XF!AXsDKkqY1zP#>&%P_^YIm7qN3 zzG=w)O-|g<>5eih5JzTzrFHwom1C$)2xv6m|KX+k!}PQIiEs`Tv^do4JsFFYbY@bs1v%VldD1NcJP?OCBb*rZ-Xvr5v93fj36)K5UVeI&%jpQ;0&mbi{=Hkd_Df z%g5}`QTpg|b!<~dZF|G~+u^TkR^QP+p^3I|!Jm;JIif@R4BBmL)y>s}Y&>L7CY2YW zj;3WT&_hYz549&Y&>7VZLsAbz3^k}?;BK3GH}rGw$D&urE9{E9<2@1EsPTdENWeGt z;-&8B&sd)kny2df>=%E~eSLzl#ZC5Hwa@c~Kj`7fBi?+QPq6*?miO+cEfJ2|+5J3H zy@-|yU+Rs&QmRZiW zuV|lM0}sZz{UfByii6>(lG0oF=*uL*#2MKUjtcmRAHdl{=FD`uWME7YHBT71nT?6=cLQtx2F)M>T62f0A-(<07vh0q&b>$ zw<@P053@yse@y~&9-cg*k2Juf=(F)n+1;urF^>{$x0cNGxjvBYHeIq9y;s^gGPnEf zw;F{yof23LGqyd}-=zh6YpThWPtn4E@4tiQW`9B;GF8ztRWXNm?hcHNq2?YR&Aso$ z!)^^em&`>{r5w)>&(l$%Mn?K<@0_!=^ripQ)xEN^;`M!P>VjFtT*;Gqs6=Na!Eke_L@b1A=mw`gKXV;Q zfCTmgV3#oj8+tAf4>{tG!ZhUUH9M(h-zl0^;uJ!9KgpIp5cj=7=$VLM6?4youwZ8z zWu)8(tS>^XsNxcJ1cn4yLCj*cZ+wC_?4}9ax><-mF*iPlFX0Rh*jIPn-Tq8A*z>lG zEh+aLLOjr&a8ED<_+HuUGDm6j$jaK7PYvm%mb_^gkgIeP#LnShG>0D8TUe*bvR(Vz=kJW19 z+Ae617`xqdzCjcpiR%1wv{{59q6lft&*{=}oWgIA@P5}Dx4>OYsfB18o!3-5ad%Cw z%JUh{@(MNWEY^?4Baz8WGa1|Y61!de;2&6FuW%R3&AN2mW^L;D^c}^WP^xeSy#RP> z4_RY){0I~Lm%go1^bX`nhcc-laY<0MH;j|#ji?i?jFQm}Y_i@=)!AN~l5UE+>OHNk z(!N^9XK7(uDhaidc-CC5)By-Awes^VCBPGio|2pWuTlTz0=xtMS5iQcg60Wi2LI2I zAVtc*mOWC6hbIspz{T8>>mnkEH6y2!S$I{GZB}7>0gI1|LKJs;xmo#m6mZ8P?=8t2kWOzVoQJ>I z-|(ImXkVc)?8Pw`Wg|-M(Z@?olGC0pG#ulm1kMlbCE%#iPTTCr$sX}@)DlC?XTvgb zgV+LLdx31}u%iNOJ&C<6bZLsctkwq9*r8_o9R3FK+(+nW@=iziXnKxEvjP@L5z=K=SU* z=;orD2)l$P982j-fr0%~0LinGp+wD7HyI&

pM}QOp0bP$b6#BMyri;gEui%BREr zuB;RkmnMp0Y%qk}U+5}+W;_(Y4FdNN^OF2N^s^L^6CfSLHZB5LsVT2yQK~IGZPB1i zrae9`1;l+cfF<~578GEP`krX~$K7BWFdJYQ86Y@?6@}oB!hXoj8WcDO2EZ?a z7`q3s!zvzqGllqsBvsT*XYI)i%nYi^&C@4e5@Asc592{Wn_w{y|9=WM&u=yuzl~RW zqttFu`^SB&QDX1CLTRjOjF>^J5PMhjR@8`6D^`?PwTTg{lt$VTqiWCCwbiH^^*HBw z^PKa%_`SPcTz|mz{hrT1eeWd$XEkRl~l8DRU-2np`Qigi~^-} zcjht*VFgw3%O4_h6OSxx*UHNmxo`iO3C&9h2GC0#L2}R1FaC@T%p$XpYMx!;J&Bi) z_*H}C*{mY76E~p%lCF%RnCNA^ zeEObZ}J%Fq_AFD<61Oi{{J!LCkVjwu?8AMsq$sgqfwhba0 z*JP0B>QtVSfeufr6W?dXA=b1xCUDl3tx$7gw;U6T6JXy0(or3BM`ziSUTjC8%E34y z-E*#Ly>Rclq{{6jmxVl%5W%mToBNFrb~scr8H|C`-AyB6{iQiRawIEZvbw^QcAgU& zji4|-e@T@&Q8xk}4bzV?t814azmQX7dn~lIo?2!jmk$D7g&{m7nZG~Gi>M_WpVX3JsV3od0(TKNJ%jsyxVt$BZbWm{jLpc8+|6U<+}_PsD;MG;jw z>uGwD6xA1uS-m5+M6nJU!)>u zD)Qw;rDZi;o$}ZZ73-WKYFx2KE-dg5Elo1Q9EO)QB3_Dq`WQ(CC?tNL2T*@5vf zr0{fqimzRBl$hLD4Zi`HGs7eJJzLtaMp^IGN5Q^AQ=WTwBiK)!zBVhX&a}W>^>Iqo zf&o@W5RB5adviR_n=xK|cZG$8ju(9W`~D_l0Y4-|)K6fyHgcSFs+-g$^A}?s^1fSe zDbAi_Fu_Osr-mt^+qfLkgdSN6jOhAn?c?6JZwzUO8tb>)_gA)Hs?~sl>Vp2oN6Wu+ zD}5djd=r1R>rn4at_8{#9vcQ4mief^G2*V+|55lvDW9<4@}4YWyo)ak&Vy8RIkpQ< z%`Esy*tjRHL*A8MLGH;;tKJgpK$?s?rR;^v0Vw)WT3r$qt&~!2Pk+kW=e)50L0=ch z)ilCZL+@N!gQ!FyJQT*MNNSktruN3B#KtBYX;%oo^w-zk>W)%9F4?i0qU%hnUV!*W zvU>vTOagt&V?TeL#b`i&%=_lr4XvqsJ@vQ!zne zYQa|F!BSNW6f_XN=&kB;89)Blu?}GyVbgVqN2BF{BGJbto9t)!a!BN)ib&G5`_I+d z&W;t^Uy7}VXLj{Bv2HuC5axciT&I_rD%CRAk99PD@>Zo~r^2+w1Yp-Ck z`#_1h@q~Ahy3f2ZybkHD-`Qcoz74V~|8}O5DG7DxYSdrzADs8BepYqb?B~*&;yqpd zQa|Y4>`-;T#HCe#RF66tq28|ufWb#W*Z{ioB?I7R(L1HAHQzE9}%C=I!4?kjjXY@H0dXz!wa^6>;JHw z{3~ODY`qnrcjc&mkDchX(oVbQnB`cs)^;k~MIv*xm`f}CYpBS0Azd-&K7P_)MAuN* z&|-v;C#UNijAmsPSB=1!yITOQRKLKh6CZ^fl>tnT4U5`y?Wu~rJp7>kZDTzFuamrx zOeLFk99vALPLjDX6chrb>tVbeEphHC{4e>_-NDk|#9T@^nrP5Xx0`zZVH?&}vDK1i zW}ES-4;G;x8uwTdq4!CnO8XWaZWu$OTB;R`rV7n9;%cAxoWWpMt}~s%AS%LhAXL^D zm%)HASKf?eIZhBs6DWOEr7Wmi8vPZGy#2gfv;4t`inejdgHTbsPpJ3N*CMRSrP`D4 zeJPx^4XL62+ztN{(QG^NO(!Da=4DM*;p_%xp4?I(34|0q)2?{eJKZ|<(WEEG>TI`* zveBxpN$B998GIl4rBC(N1UvthG40)y1+HFdv7-{;2%W>f*n7_$97|%De*7oV7AcCM z=E~&h>Fm*Ep*R)BTA=7495R?kOca}Fn)fuOrF2fg+Vy7DqD*auwXlGDDQD8FlxxC2oK%`1~@ zSCOVqmq(J0S01*=9`07)l{(t-e^d)H87=!dmss8f?I4{w{e( z$Fsk3($G{EKwr2Zy$^uu`-`0pVF$@)3t7om;cwGY^uOTi031T6hQRGI7vIi!mr!wzomSq* z94|DbJ2>oU`w=vcRJA2LKF)q7AO;8;_JI8bOm|pBgOi3|s~|G>e7nEyZ%!@)*&i0? z9SJ~rJ!kgf(ZgmlxYC4fz>e3r=0dlwd_3Cjb-8+%!YAkPzdr?h*5#rpb!eQFdtl>P zLGCA9;C^jLe29qa8%|A;NGBgbPo2Cyu7bj+S)ev0Pk`#RY7=`0;SedzY$jB9q!}uU z5RN++V|Dfp$U%O@8fZ&%A87_ibA+_!hZ)r~LRsOA+(ka(zH;l>xqRl|VZUF-iYp@uv{6cuU={VyV> zAeCK&x${t4NyG^J|wl6mul*1YJQ@i?xFA#8#;=%X*Q-aPx~0wLy>9z zV3i&3=5$H<&p$pc8#ATwxeh_9`&>0$h?G1bp?$S4^K*{{-VRU-Fmrqono1y?(Z)-n z>Yo22+AFrTFg!I^%HjeCcfQ0bB8!)SJF-1KnZD_)@e0oc3acMDzA+qi|HGDJWaALs z>Pxp+ae0}(IQo0P1;-Te97hkD2?K=-kGW0F*Z|pk4TU1Vd;!!KV->*B3UF>Sd+;> zcn=WXTWS@T=XcMC;5wB&d#F0$tmMd)A(cOtwJPUzGvvn!9(a_vNhbXZL?$BwmP<*j_vH&wrzCGj;)Sut7AJ~pL_4af5!M9YV0-ELp|(O zt7_JqtKugRv-%P1hTxK59yl8Z5R>*n*pmW4$-y|7SsMLrH9={fkr$LqSf*rnJQ~nL4{TnHkysXJv2v6PA@J=mFnWB#i_Q?0YNt6UY}BW_8i1 zm`sLyeMZsF*Kf_PuS?Fag0I@N$wv?_fQyw&Kmhjt+<2^AXvo^H2_bhsX`B;Xo0~t< zYk8M1h@H`6v{pj(#;YKWeF?fen%M|RTZ&lH1lQfL@YY^st=4;>Q~3*0;lqH&OQ}}_ zaGwSE1~9fAH0Xktv2+6b*IV~pyk;X>OoEzOHvV*cEsatDvz_b1G0^UX8U(FU0ASDN-7kD-$!DFxnBZo1^l>J*o?E3d{h z(-V8`yN%7$`Q?zdcJTn>K9qvW=~j6ItS0QB8a_EDVVFB>}t z+0-cHTB_V;@X74jE+=M+l}B>zsa4@zp}4aT+y~i$sU$4f6vwg(&l)FD*)go4o9=X6MJH{oqjt4^W?vFI zf;!JGcJDE@BdPDV(PK!t+zSHHCpH*;W6 z+me+oO>T~0_+z(0E>%x5=zk;9Q5BZ$bdbK#{bubZUyUxMS_-uPZrPIx>H8v>+r+Vw z{6!f{3F$#lkxJ{_b)CCBepyi^BQ4)?k;i0lW^`Bnng>KRU_N21Y%(X{{|G5AU;}y| zI_jEp-A!Wl-2_m5$d!;q2*(*GQZlgi<@!o^K}^q!xPk2HFN z0$f|646%BjCd$%`Xuday0Me92OKtRDP5Vo!o|ql*XQm!Xdbkf2SbWW5a(BCzO+Kd! z{E$!gbXp3$GGnogR>R%|q(vo#k05%?x4u+V@|^n;h}azj*mJffq2+jGnJFoHr1{q-aFW z)|XF^?K@CbuK&5y-2c9Qx0t{^sJiqS5ws6>3+8%_sNDdfboRX}{KXrQgiHf{*{Q#) zED%Z>Zaao9?n^E>ccxGP`dI*53@t?F+5B7W-4N6Orb`#0(q4m-;>zBm=DqgQbV!R_ zWm4k$LSnNbHf}!Vm^s&lO+hK2uY0>HsKd`OYWw^4_pc0c>~^n2>pDQ~*7DZow>K@G z=T=~Cy$s=x?zfW|sTMh}SOQpHouZ%^R!&Lz*j$l6vd-(XBh4LcVj=k?$Q2L5u7u6? z>fBZIn$3jWwco0*HespYg&g4yUb;bgEyvUIJb!VzL7doZC#03NL2VEt!?Te6g>IXU-BeP=>(`L+&MSP$^ zF_2H7!D;OO;zH(bk5j1`KEc1ajXuHNKdTc{YDl6Cdv3MDkQ;j`tD!%~I*2u%2`TzIi1K~VsfjkPp`IWVKbyPL{C z6>_EVGMHYOQ;{qD=<11`_NKGLiYjO0(SCyeijrN%hxJ`Z)#0at>i0DjPSkU%3}XSy z@)?ohKMF6YtQ;EAl%JK)Jn}G|2!Q;?0jdf2*W0`&W#)9op`RWD4|uKL)-rCW|`m9Xp_eV>!9 zCSta)4~2D8W)R8qok-*b9K4nvR0BPhR__<0yb_F_N%Z8s7yU z==*EBqWYJubX>o8sJCrVPNy!js?ECU*}2%px<*LuB=#KeRd%h+7^OQFne0H(I;^)_ zF^N!Qm}IAzXp6tQa}vKG=7Cq>{aY2HW3UGKYxP&E14Wb>p+!LEZ+S{Z?H0dVt9ga+ zW31}=lP}*jV=0wmrQgYHw(;IEY^WmE6UpbKTF;g=nPjbp^80RlJ17r))y4;jh)inC zOy^B5qa#v4bsvJKJZGfqXxnf-r|d$Ko;;kBHTiK#J#K?%mdXRe&*KZ#EA!Ok#<2j) zhq`VJtOHd%QJd@b%|GF+rOh;>(&BWlnu3Dq3I&JWudhqI617;2{!g9LovpJ>8AYNXTyuX#KS`uUlzL%Cv2 z=9W|g!;wD?bZZ6QL601~qtgoBK+)1xZ$ZBU%*T_`nW7vS{Uu?Oo}ozC?hjq^DPLHI;{+ay=I4BbN~SgWfnr}PR^=Qb`^ z^672d_ok;`igz0ukIPry50_vx!GwGXYX+*vb<4w4)?Q!|7e9bFA%`_BKT9ZVww)J{ zYmW_*+Fg)<(b6(OV95P_CQv}^n8n`&$5b2l&9r8WXT_X3I=?rT7hdC!CD9Jbu$1{Ro9I*^1OKx3iNH@84|J)#>P`>%Wl+!PJ5PB1m^+C1`7rmM~QY- zC0~IZ4y-mfZRfJu}Q98Dhhs|kd_sr@b`p1Y}= zCq0d=lJ*c#O*T&LbyGa(Dz8YPp_+A!m$yY+kQv8R^}+O{(y<(caNtvjV6GP((;SnB zBt4>36mG!({mYl9CHSkLO*Kvd+*Lt|iv2m7SRKg`$UfCxI-5S+U4Y}c@Y=ud&FY(k z_ApHTn4y@b+s+g5;=d2Ma_V4f?=GLcw~Jg!n)MoT>XO}|e=yw40o%p}35ro$Weep^ zw7~rJQPuFHxt_@|nZI4EMjlU7w!=dpIl^pKewlpeZec;@slVR#d2dCmF)CZ1=)38v zz0=J+K%O}!u$R4CH5eCVhtC|QtxfM%rPt(fkM6gAvFqu)q-&YL(1^%Nt}~GiwO`rY ztVB9QEZ0@g0K?nPW3s3v>cof0U&aDYFOfm>y!X>r)n_(H*jI&M`z4#b63@kcGQaz} zCeA)UH^c1E-~ns<^H)_1H0iT|YWER&OQ}LBV0N7)|4?s`ca&Sif$uO@&$AKblJUZI z%4wO%&m5cclTVoKT*y+7T6X&(FC9BRhe~NLFXU*&9JJ-|w6mgz{p`&8r?d>T+ zuX_vuan^kIJ+n8XSIOQ9jDbU4N7q$fAR;?7>f9J^QYO|#{3^PFyi)!^mNOkX>#DQm z8XeVOMEHF?+o1B^JjPQxkq=GXdfwBCSE3zm&<~`Fyj{fi#3#;e7|ZJW?9lp`Qnz8X zbC)}VSuGLQgh$I@FOb}E46>IxaOmsq_j)0ilyfokzgcYL5tIU8Vq*Uivab&z#WO(;AH+bYhN?2Cvr!9!l2-&*}Lkklm%;5a=Z42F}NbaK~dw!(K9g5u4QUj8|pQfN3 z095>6D^W^59lwWalWUWK7*y3x?MRI>xWm5PKTSFW7+UIm8U|EINCwXLd5SGkUT_FF zIn9X*Z#Y?9oDH0d3(8z9fK0i?Rc zOe(D^MR|D@bIN1clbJ~~3X7N=U0^Y1c^uq_V97P7iK5x~KpP0`r1+$HtE=R{Lvb_C zj!;XEFFteL%G%*-?N*mpAB_&u1@2GvAR1bKItCmOY9%Dnw7t2F^+V?ui1yf_Ws`b1 zDq|w3bQH`LGc5cq6-GQ`Y0%761~OK-LQ38W&(b#p27Q(r8u zqWo8ObMzRoGb4{j8LGOd8K>;F^G6S?v3{x`!3d^qWk=i6?(iH>rTEoXx%w$wL2LqP zOS{Z=mwX1=c*cCzx}}vH z{~W#YT@<)q+z~Mw8DQsVh_t;irWhM)% z3W0U0hYi}9aip@aZaN84kntw+00z7IFP5aEU8ezvp^()%9tJ(?OJK8!t2vq(+JVV) zMG_6ubZj;F)f?Pl9ZkBSKB5diAS753O@A=lP@(|42TiD6F2rrFl6s&2$gs`9=~a!1 zk8?zJeaX>hQF+c|bE?XuSVX>}F^jNPvVF|pE5>1{x9R4u#-*PH4ycL0Z@l(m{htWO zeUFJ*UxV=<_I@DlTc8)_|49a105==!f9HWQJ3MCit|!`iu%g5TL~LiO8SoSO5PNZ? zinLrW%g-;x$V78CG^owd7QCUG2Ea0qm)!+P;gZ8`(rb! z#>+PdR#=o+dA$gi@25$Ple$jb&);8lAY3~q8;~+GXU8{%uFX#gNXDF(zuGd~(K4BQxD}A!bFjg{3Mr0gs3q?m6%HD? z0zi91&~(i!s#UkH0`|&gu{4R982bJ2sRm&Y{J3|+26tdYhoQGiV3ZzWYhYtVv|#GB zca+R&SVwjC-?R!*lD7uHXCpQ1vzBASY-S`vRDrE0+QovFj@*el>%UHNj$T{tqX)}e zhK@nDI#UuR$GPJW5DNA449e2=>COID^Z1jrhpG-Iwk(c40##Po*~db!;LA$%=(!E* zMV;EFB~k8(Z2U(c75j{+eQ|{iw*8(XfAKw`<>jtBJ^={8{zA>@N$i`sWGNI`F zCDXp24W#o@*1XO@*UNMs6ZY&1P4S$GR8VkPMdQ!lH)@+@Fw=NLXL&(x?_^SNH@Z`qqDzh7F7JMX zvk)9M$jy5j!&3&Wu`v^h$AL3wrQzCWyplJEVlYv>APYT5b`5~nBngdvlX4h4$P1hJ z>e8AjPF42O}n0S&o#}a$NCi4*OuQVqcL*D zD`BnVe`Z@;6?wZ!izGcExY*X+>-#^5UpgZ?+hOJz2o^tX56MeSl73dVoeH2=%Kj$D zE5a0cN#$+ctyDytqM2J{yH5Tq{9BailoFr7AL@k&QhQcUX+Vk$=Fw8!GPXhdw$R67 zx%z9h!L4Pv&l`k#Uk)#imzUxq6B7jb_UDl`iFa^JY~YEK&qL`2iCK z3Q{aWTY44fXDR8fntG)j!RU_1m`5m;R{J~6QdgYi85^9RaIolvD(BZh5xq=pj8K^4 zhYLETBXAeKO}PxN-RWN7xxs zy}NLuZwr{of}ocHxws2A-^N3p#nR_9A5%_Wxc%0BF^SxJlzOa}KUxw|;OPU!fGU?k z&>;9he?$c4UP`9Uhvs;I92(=)9TOa!l?EGowO;f%ECh{SlC3kFY)spyQKQp1w~y~} z485a0e5R!&O(py@aoZGWk-BE~+v`BNgJ_2P^u@Ais!(HsSq`~O<`_O+{=o|VK4_te z1TOAzy|PmLdD`PDVhzH?pDaITAY11Ki-<8^>0R1EO%&2R9mc6iO$1J@6qw>Dh&_o53y`apFy=lYT=FJXK$*E89qc8} zXQSLNpI^;Hsp%f9%l-p)yjK+DPq_j-Z+JTJ zE~!jpczO1CGiSuxLW6W0P*XRjJTEw_c%9SIsp2YE35|E6i1&KwR5cK!;(~?yXQmX{ zJWF7zLGHBt`|o4KS@vQS(Ddo`caH)WqpW|OZci!5ywoV=mdsb@*T94z;W%_nn8Wtc)pVw-wFP_y9s+ikjuEa!!$uCFs`&%I50Gz7L6BvYug|}aK?}~B1vkI?jLd{ zm?1EBR3Nf3bX>W9s=rG&FXayeYu(d7EkBc^bhQEUh;GuMf*=oqC=yA&MGcyNEsi2# z9mk!rCXC;;7I9~|1DpF6 zV-~;|Adi}@pK*`to1{y>HOb@6K4>iRt3vu0R-VbdU;;kxDLvi7<#|qwK#sR_1Nw=( z_TAj(&FSG(r$-EAp9ne2@1#{G?c*+(Ew5{H_vbbUu2Pr*%xlDeJ&+Sbf1%4$KVvOK zLMDO${J-1tWt%#8KcAr3HZ!*Z*D`D(W-)*n5q2p1cpJ<<*tI+Ibco}of^^tP>NJSn z4?mICnsBY$xF;D%$ELi3;vgGgS(m;osS&kMGcznzg{;Cc0>ifWEdu6 zMXQE&!rC^A)XWd;`OrgRP$k2`&}x~jInCp;PK#SZrFOMWyeD=%P=Vj=X|S=NXaW!u z!IdW9$*cLL)NFqUX$8zXI(3g#*|-3DK?jvXsocmOKDfVqs+UXn%Q?#>=jIBj ztvb@hvfhsb>IFPm9+T)bqb+c(&KbE`PU`!GcHH+peG|h*yn$Eq)K6BTm*di|cSW}p}nxz_Zi5(t)i9Iti zwYBc3A34)>5y6&|%CGU=x0E5v40Ix-KVs^rJY7E&`F zn1&W?RIKKK5ZT@LtbE1u$^$_zoSxW1y+$?$X@Baxw5AeIicy1O=bDur_0(o`Bsaoh zC^(cbsQ1jGPn@DC3SF^=>$w)u(`LD^wU0xrh-}|0c4ZD}m=#-`G?iCVX;P+Z9e-Fd z&>8I{I*@OE7o)WKSSM(g?Q%hqr=RY#93_NOb3graJcDeJWIbB*cl^3IzkQw1y&}r- z_64d!jDy;HpMeWzJPLkJvo}q3XX|q_;}Mfy%{BtOv_<|ESphkVJ2Qi~UC4b2Z!^X# zaar06-(11TKPxFW#1o@A{vCd}lPvCCjyYYKJEN_XCL>-y->)b4H z9L`fF2^ZvO@!%=>;Z$?^=~U|Z!BW!kq;uRSLN3<=a6qB1zfAmgRk*^Ig}iDX%Wgh8 zxEcH2cSUs#Vr2^QLds%sn7XDq3;IJ$WHM;Y;giV9*wFlG#X<%Y&yhRL|nRrq@;EOSwI=B#^;$Ky_; zh059LMVKz>ZcTlU`;CBa-oWy9&boPkL~^MOQTS};G<9y7r=a+i;NRtEjsH1oP&StT zh%x{>C-?ui9nwqIi#q$yxxdjmwTBHDv(`()k}j-8I&^F5akVzb*zK;QYBD}<=ecdQ z(b1Ok<%~^svi_mF{cziEF4BCz@%42m;%e2~->m6*TZ>5g-K0k@&1TCFxwrkQjWA34 zrG?N_S%nt0xxH`qblYX)3%Hhdtlz~w-f{@^5Xx4F?@u}KcOxk%?tDC6`{8Z6Zs&N{ zYm1A}>6ebS&h|u=RiFO2Tz@}rwa>Qy9p&Nm2kHI5fhZKXJnK*zob~#%Oa8O!ZFn=Z z(yjZ9fkAs{-hy8J@Lo&umv#3F`|irAZjV~-=Hdb2h-`aq1S)bs3qU+n3~xEsw2&Vx zjV2W&f<9nMJ{riPIBqH$8cmImi>DZ#$ZFQt0s*1Z*96m?EC<1tgl#IUs9HD$9yty= z7-<@y1tZc8YO1`#71VT6rcP`+Gm6Y+X*#MX+m{pX86N24iMWS2 zP70|t0~zcLQOVH82smZ(theQ%qg!1Pj~SZ$fK6K35LQ+!mPMw}Yk-7pWM_tU#f1@p z99SCz@8^!k4gMFccR$;ErX(?YLmPrUf6z~#_Q#h4G zk~@hrrdOZ7f|T$2)~91eEa(56&7Mw!LA)TgL2r$c)FzT8qDompY#JZQXwv3->j*{U zzI2jT-7L0vX$pWwA_=j%;|$pVdOCbK(I&P1WK8$v(m zu5a7@1XGTd zL7l^GJ}L%nhEoDiB-NT^f~$h>CTI|qsE3ARm4yp4{zjq~z>Z^$6eCazr+lXtzfeY1 z9|lXamvwT~q(su|Mjg_l=Uteq5D`x%fl*{$Ox*cwQxy}+aY{Tz5Szd9`HM< zS<-&}XqEleZt@+hzO|11+&~pK_k#02D5n1nNf@jN8rCe$#0U%pl#T6w?m7zx+kc&f zV&BhTCgiR+4Cf7H%$Ris`f$Z$h=LX~S0w&nQx`C6RAav9C;38;6CqH0^R29rsHn>7 z;yUCn>>=Ey8MrY7g`XVo1p~sP;l;fWjd-wvcongGg{7}ivCxhWIxWkOFG<=Y)M<}G4KY5RuAZ6`_WZrh>FmqW9mZ$>N1qieo9H_nWq25mMv*#2HAMS2I_G}T|lqoCh zhmNb6LQxxMgEE^-5{>jmxm~fjcHz%)M-0JueltRT&@l4 zpNjnau+3fi%JUc)oAuAP=hB*0ja&dCS#w6G`<}y0@XT?8IrgH84bWftdv^WyvYJLY zrh4cI_wbz}@AXrdeoP5l8~NIeVlG8bT+|@B7*;FEjp>LMIn1RSG{*Gd>i1#Mxf>$@ zH#FgXcl%-m86}6-pp^>eS*-_MLI<^t4;D$X(9Y3h@**%X1mg|WKkf_5UG0!E&7(a- zD3c$LLDGyTEJy zktEwfs~Y^bCUr2+6*NgRmUGckEFHa$#09(6Ve!I-N3JAcMaf%C@$%@SUXSX{2UK}L zH|PKS@w6}uFp5+HFnUln?p8(uumnO7mb5-zFl7+dR&+iv#P6W&9RI@hdz!Qn7%dJv zCovQ8e;)eq@iEGo*;%+)G7~eiva+zJ%|3$S0qh*C{}tO8+MW(NBc9uvPY&_KQlP}W zV5l#>P=Qc{y*Pu1b4^y1NscVGdxCq?{55BCrJ!#Y-B9s~)RI8G8RaE9ts(IH z?ZW-46)7KC0vc!L4LM93QJ%eZ=n1HWH}8hO^0?gyMK<8bk$}DmaG%&X%P4WKcrp+P zk0vo~%F*g%I{sR=Pe-AVCQ-ewd2u0CTX{mRzOoAV`1DMl@vCuw9KK_6n_{%hwts6k zCyLG!E5DxGlVQ0jAp?NTSVdoIk<2^Uh`TBul^aHsL~x@=7u!A-SSD{KIfPiM}&k`oc0a%Lg;V9rCw(jlp9B}7DK4!l&=2y z1Y|F8oJO4ui^f{gL0?QPXlOzC8i?k24q%-7jplUKD`jkzx#wZK z9RuQU`Ij<+VOL6xV2^9kN{by$(YmERX=shXWxm(xrAu!O|Bt6}>(iMYn}>3j@%Jd5 z?#+`?HGIg9q8%x${U_8*kd7j59vtbw=vXl*<3OfYJ{kc&8tbxH$wRl_8^}@9FjqDN=ONtA_%vqsy|u(yNYau6nN!k5M6te9vlu&$)vIeU|NP!BLWwnV7y<-{On z%vniBL(pcqCZSzw;zZyX8rxfJD!D&(kaD{lE4BobYXp4dk%j;QJ3hvK0Lu|#1qvU8IhQJdn~rTS4Jvu#fdt8uRxqryA11Ql;PfL%kUxi;Oh)L#`1`9Hpwi( z@S_!CgJf8;LR8RBN*=*>P{g6e4GFOnMyA0Dk^ln;N6F-OP!D1lr^7u5-IZuKCuXic zWN9)*^Rw`Ik%QR{%=EcWqZC&64lXV-8BM%~Tm46Wlp#}scLR6mg2kI-)am zqEZnPjJ_r@!4nIt$_h6xzw2F_p%_eyv~>!vqYt|n=h+06444}1O(dBCpv~=(7WF{W zg@IAhz}9}2v)`f2=JgFyR6(P?E~*SDqW%6h{s*+^B)cLa_=7;7X@5C(h)C8=wASC$ zJco7bK6x|4tf7m98fD1&El9so-}dBPO9K)!k2MV$9E6Li;^@>zH|nCI4?^mqvJZV~ z6`?l?>B$$prQyDT!kv^Gcz$BScB_#>dZ6do&TO{JWH!Y>R|T``J2~E_eQ!5zT`lfC zYOlZ=?>}1(^lifG`?vmQ%K^Sk?*FV7>dkWd+vBW$Vz7Q{DIv^Y=AH<)F&*NJNr+>kC_^!1{9C+L@6pj-v7*}jDPtkZ;ZC7Mstf8R%hkw(;myyn%Ch(Hmd)7)m ze(@-e?vTer5mSt+Q+kAuc-{7;L2+<~4U$k{D7BI1H;8awo7m8UrMZbjG>nx94T~;T zo0vKYujFjqM+VOPhI#L>11M2CVHq>RaPrX)xae{F24????SQ|z?(J0?lW0plC?<(Z z&?EMvAZ~jS?l+}VKS9Ebs1#0ypiyg5>;@A!WMek)K9LL2Q(f&5gR6kU1Q`;!$iZH( zi-(sd%Mn(BgA0RYlz1X&>`2j9n#lAX2P@OGnhBr(cJ@OyRNq(r0Eiz0qr7fq8**!q zB1KiHV)X5|d>6162P}EcFuZohOV+BAKo@DTKy3-45zjj}Q+6&=mwpzDWv;)uS)xvb z&?pdrT(WUvZ3$4J>z@q?x3l5r)D5Ii-w$`DQQaJ>DKrkPpo=`&5}yLBGB+0?pZitanGV^q=!5mRq#|L|z54a#iV)#3LOAi#CW1lpq z_*R!OkZk^~m%OCFFmg_jN#2Tzi&QVHavAA_iggKrC%VC8dN5J119*oX=f~C2ouq; zhAHM?98U-B0=*J8Bis;k1V}XTiFr^0G<^^d%7Ia^#o#?qOlx2oVe^&i_s(>44<-v0*Qry=spqqB(2&*<~^N_bZ1RA#3^B$ zC6*lZs-RZQzp&u%pZ+~Xo8OoE(W5{7i0Y}W2`>Ee-n||dmK|x{{v10<3bW3>_;~47 z0-Wgk{fKdx>yPO)(Zi%b^ntbI#+s48021`kyenRgdGuSmBIwMzDOR}1V|m?_v4 z+!jSsmQ^z+QW1SNQ+|Ew+Q#+C>DBQTD0Mp!&u*gyiteTsc z3Qe6%bNnLkt#)&aiShnA+vIs7%?B8!fY7gK4h)zzBh(@j7hB8~VxRDjd&QGtcouDR zlF4cghd}k}`qhcaYl^&v%<;;TEI)Qhs2vmJ?qy73xWvF1+(}1|P^g@}qHtukA}V#w zaK(a>85_251>Fj8?*x`1qt^J`O^PvvDWM<>@q;Ay$X zDOcC__ru2R@ehM^^Bx?3xok5QydVZ_G{hvfn6qfeJ3i>6HgL%tC4X4C>p5JS4b9^n zA0Cv>z{a6fgEsx8uJcp*Z~NzeTFy02u$+gnKF|%fYHv0_UgyC#J*^`@=nBs6%9v*I zGj|AQLh5`yAUm^qT^b>+@A(l59jfH7(tW5=Xw|il!n3Byp%ClA0;d;Q*&@j;dcVo+<}YDlSK4 zyAxTLTZ6}b*Mb!*CkHZMpX&(LkJc+`9~|wk8Pg@Co1f>hO>P12xbd~3K&&ABs70-ArqO)&5@YFfU>AS8l^kw5CY7> z8elDQl6XLs{^Vm@s)dZ-@$ALD5FB1K2i1oL2pkI(IGtFs9RkFFgv}>U)x&eqN7%4! zi^P}A&+`h;M>MRn)ehaO5$dCYu<5E1T4%&^IAl&jorw2 zo5=rhI-5~(77e)h<1u?}Sff#4vAyjMjsuzMZ*XIupf~Um4Ia?oHLFI%@gMX zypjn`*#?AVD76T$#J$CmHcgjsXg1ArVlhLE3#+ckK%}e;{7<1AF}}LWPhj>-42=dp z6`W0?@{w|9a;WBr5O{f>QoXaroa-x&Y$Qz!OXf@*AKdqPtOb@hZ8$hrF!H!twQm5H zJnmUbhjc>#LBpE7Qv#`?IOu*)(j}jivq}wBF2*Ua6qgr878*Z-c%Ei3v#WRL2tCK^ zOwo&CaP9RQ7kj=~r#?6O;dWE4G~_qvc4++DL?j*9+pL@#rKW%T>`9< zR692onx17Ec7EULde@tSXBFo^vq-^6(e7?LORy!tuReY-j(7N}BHk54kdm8l9Or?V zW5iJH7=PTU>h`H|5rr$ca-?x_X(j(6$~~`m>nl4-*I(^872e%t%?zvJHU;`BT92^$ z)D6-IdDDJDo#hh(c(_|@#_k)m4jPLM+u_{TC+@dAU7*ahV!!e=RfU-P$WpKoj%mhL zl@J?&NHfRPpPpRUm_1%Wr{hCjz~y79_J^4X%MOxw8PPc~J+`kw#SM*8N{ zr}Z}7sD`E&+p%i0dKHiLp!&G7B!7<D@tvwm7P^@Hgk2M#QhlTclvoy(O3ge5?v7GrF+_ZJqH(xeyGzPADx4r zbJoVRzNxYqL#x2<=FS$kz`4GTKgjeCqUCvb&A#e={8{}oI$Bpo?Y*d&&PxOkfp11C z$f2Go}6W7c_pQtEg)98|IEQXFk6l z-`kJD>uZXMWzg`UM&hd2E!ZWNHqJ4Er+;8L4q|eN#6oZ0_KBiv-S+$xwNkTJ;xCp* z{#MCWL4=gNYhD%W9vmUQX6`D0g+|akur*q4zq8|K1$+5OSiiBfq4~E(72b^?yaT`4 z`|-Ys&C?d+!Ls16>;v-H!v3oxev z1j#2gc0oF!7ol<>`Jqza?OX{N6vCmV@)Ai^O*t~0Ik};}d@$kO$qamrIv?7D^z^nE z#eJ4f2KYYxmgM1Awcp8J)xScA-}Fn4rc&>>f+xKr_JI4P?%vn-yztiNbJk4Copr>3 zGOof;d3ag?E~soC%O1GV!N{aE+)l{>*vR4%;z%et=CapbDP9F{Nsm{YKY8~cZhR+P zhEnYFh^~>gxCOp)CYER4{_?)FeEfR)s@;~M(@}ilRkDBQU-16%7@|1sdxy~tbZ(*M zoo~OY>n|&Kp!XQKAvIhHJe3CBY*}4J*@bQ%?$SIW-<29{>;juFNdcS|l&2l7?;knk zDE#H0xyC)8M?Qf)F|dC7{3_)cd|wW@DDW?-fH<2r`D`==ycd)VVC!9`f@Hrt}Tw9ZBe z|DE+3uvufZ4r~u|NCHH}2K^wW!j0z|K?=`=x5DMa8qpksjRpjjH%_0xb?osMCu69$ zneGrcMu7>{LnK^0Tw2wNzl}#; zWR#mB*;(jsnZBWNSObvL^s=W-${P!{XeD--D(ri500zUkJdUm+T@v+$6~kH)ZYp6|t|crYaG4PHjJyOZysGfQ)8M*&DZ)lNUI|KRN__P(PbS$$i)%~MPBv)2hQcvH9hcOd{ zpa~&VYKcq{6^Y z0QWVhZl6?sr4MHO40YYaMgU9xb$`iYC_i^mPKr@s>@c18TKYrjCmrxI<)>?PJf z--?~_Z0I4BQ}Yc(EsX3S-ksf%!i+j00VQoCUT1;Fe!k5fNV1T(9GB!}WS$s`0{1>| zf9aCf>i$?ZsnS#iW`c^64hY9UxtmFnomBI8s0<{I1una*;ta3LSm>6(Wc4GnaciY!lMBR;r2sg7h$<#Y;6d90 z<+?%7!teum6o37Di~9tbM~pz6etNVy)2E``@HpV5`-fPMO7l7lDUTo1E<|K=aJVGy zbosvQp6pdYFR3(h`E>C#&XQG6p+;Tq|M93oT9{=`uSteWchY7K?%h+5htY?A)y%9$@ca|0{tL`hqe4)2}H0 zLp~#=1^a^GgL1I77W#s5W5F{qN?18LyAU%oadQ1H`^?Jr-^$8`wv|1eB~+l3n5*W zBs*ATs4g*Ura~qRa1!!C7$LhVMhC_dqQJ1p#wi91sXzySnnWhaR-~h1=f|^2A(+9d zQ3p|kPy`W*Se4RE4zx93yZ8g4u8CR;yuZ?e3WHi$N{wJ(5DKWs4|Eh^x!Fme9Lh?HXUPT< zou&u~{S$XiicQ5JkRTlgx<&LM3Lw3s>jnkO=fFZqm}qQAO$O8WU@HNVxLy%F4|GsO zx_0o$HHmcYdT@qad0rbC(OSu1#*oE4RMWlu1av4d zII(Tn{^WjAQhSNTki|5Xiik|GrnnBXRQaTQW-u-(;Tpm-*ow8+f`&iQLn`oDGo=uh zaKAy*4wF*v1l9-Rjw1pfFoPyknr0DS;ArHITFkQ0R)dUkNxtSH@!=4PpjRG!RSUIsDn#WFsKb zfzWaj=17a_SH{1N{J%3W2T;+n8r?styc&c)Fcy???g9xJ7+$xh&vxb8vG03&I?uLK ziLgHYj_eiqWDG`;|GJBcs#U=GoO^TwyD*s51XQqkN_L;@uRhfgD;{1GSSx^iU2fy? zZi{M%cR_*@dMx0g&uG={OD*W_OKdHUe}?5rFg5pd;KSw70qW4Wp2{NW)9j@HH}y)t z%Mx8*BW}@Hfa`a4SXY)fvyQ37HU?2;n6c6#T3=)5m)?!09ra53P>Z(tgB`}SO|;L5 zysW5=r!w9T9iF8N?bM8i->x(R1$)Jun~xMoJo7jTQq?c&yKl=1Wn365PrnC!2&vnSQAOjAp ziiO3#mnMI*gJv_TgB&mUV@i~<_GDwcxjwtrUkAeAP zOiOc-ZY0WBb(%F{YGdSL-a2T%HwT(_Rv2KrmR%fWdpM058~&;4D$U9UTeusit>O@0 z`FaI>(lQ_)-=u^JrrG=(a=WXA9KURmA&{!hXK!&R)F?pEzV)XeRHlmv-l6B>1T+zH*OPJN!wrXkkeofk>$`z zzK00c=Lk17V>`LTW;c(o;NyLks%~e+WDa@Lf2RxXyK-JHXTuUo$<^(rX^9K$1O0DS zP8RVsFEzZxEg9wcpndr}YD{-DwsovJYAtlN);V|L%7?HMmhWIq zf6I>6beEKA)76^pV$FB8=AI5fdS+?WXK96H+I9%bh$CS&JIFdL!@F3sU9EMQ0VZ^{ z)`c8acC=>2(*a1^5=%P-OWO>y{FEVKn%jh0-5z`+o7+W-EAPK4M^*8n{y3nrvhtjM z362M-)oajMSuE<=v-|S?o^w6q+zQT(e}*)Wo^#1LcNXQ4+G3ffXItWrbkWzUi~eo3 z7?&$sqee&J&o}+cWS4A2Unc1|*|e>6+_s5q0&(AIWu} z`%Pb`Htk#e$Zs>YwNA?()vbIxv+2KzV?Fj0F=_fW5oO)9$x)J&|J9i7N){=We?1#^ zlefJ#*Rm7Ga?yXjT>QELoEk>k2J~Gsc-2Z)E8tZ*8Xd*Aq z{+oJK_J5d{Z6?jRJeA9M}==!1XP5r!0^W@IKEpOLl(QW6t|GOwG_Ni zyR|d|p?7O(7?}6z1xfs6pHdtbmi3Yp*P55bq8Qf?^o~K{;Hz@waKQDse>MYSd2Y726@58<+s;9rih&E7F4>EnMucjl)S8(xRW_x%QCW=7?<|z08du ziQwU129+DF2HrTx5cQ0;e~_BKQOH5<=*voh(}T?|#;>i*twGs}+&aVrn~n>N6;h}H zeUB5LrNuqPBCjo7JfGKy)AJbqz);JA8f6{j=NI1}nd&Aw=Fu0m3PC#viiG6C06c#1b4m znC1aJ9f(M`Ltv?R9&BW`5CM(|N-D2r40>qlsS*^JhG1r7r3#cbnhDUu&aM%k5&;sB zVCXtRMKb_;66tOzgI#9eb%3-n)y5)~fD*HEkRX5^k_u@hf7WY_8HRR1ZG)x22^`Mp z}1k5to+hODa=??7t}HWo&(abN%D>&DU4ethm7utoN>b zSdNQX?0T7g9l|Lkex?)8Ti2I2pXQ75@p^J!!wuA{`C|6z?D?p^E3^LF*{GaV*dMM( zIBr#oPZ|7Af2ZT}kxpM&bo-Y-F>7uAr}&XjWAe6sb$$7!nD$q_TBon=K>E+;L$VZ< zq04oA?n!IFny)zk6R*G)7$!o zNd@f^uEig$e9ggI=y3tjv^UF6g0a&!6Z>sQx1{!{MuNu$3Y zX>5q3F~2KmWSca0MH(HyV8q#Ci!?poum<%0XJ~UOvupC$J@U9Gkmr<(E)+_?lx}lK zb>vVhjO(8-Z{OX1{Tla~3EfC$lQ+fU*R7@Xe_!Lv9udy`5frV#9B6s3|6OJxB$1=I*irQUrtbPJ}j+yVmm9&3sbHy~i{{a|t)DM?o zVFMJG{CEQke^hyJWiMD#Wo9o)L|Z&DIX5*qH(_EjHa1~lHDzHiHDqHsH8x~7V>B~j zVlrYrJTW;pH90q7Vlp;1VPQ38VK6mhV>vZ8WH)0pGh$*gVm@6jQg3f`3NausAW|<$ zZf0*FG9WM@QZG|!dSxInHa0I;TQMLrATnJqMrmwxmv$2a7k@G_K0XR_baG{3Z3=kW zHPgvXR8bIs;i`L3TN?#i!3Jdz!~sM>8AS!eMjTKSL~&>w0C7NNQd$&b<<5|>Ff8CD zSm~p%bW0+{kVs+(^!Zb4zEu8GIh%W~h{(o9AQG0hIDlQ4hNC!)WB5Tu(nOd=-SmRZ z4^)Cln-w48IDaNo+=QJ5_*f-WYQQWc2?7<6o3~ypO-oiG#jeGG9w&QV(;C~4$#uD6z`>_;H;c3jkGCYf| zn2AMr66^5*9>p_w7i%zzSy+zOumQ929yVe%9>iKahjn-e&*MeBgiV-(xp*04*o=92 z1zYec=3@okz)n1jmDqvTu?h>Y5Rc$}e1nhhDfZ!Ob?LMIkBI#8W;A&-|9PV^`|cz7 zPF>cz?=;zM)<5EVbv3_zudTHH2?y21W4_0KSpTAKc+NS?^kt+M!%Vu@y^eS aYy#!9NXnOIr2`%dH!?9a3MC~)PeuyCJh@c>