From e35f8a6deee7ae00a3d63e7acdadabee8b125649 Mon Sep 17 00:00:00 2001 From: coolneng Date: Mon, 28 Jun 2021 19:01:25 +0200 Subject: [PATCH] Add DNA sequencing history --- Dissertation.org | 26 +++++++++--------- Dissertation.pdf | Bin 115338 -> 120653 bytes assets/bibliography.bib | 58 ++++++++++++++++++++++++++++++++++++++++ 3 files changed, 70 insertions(+), 14 deletions(-) diff --git a/Dissertation.org b/Dissertation.org index 090b7fa..ce69c8a 100644 --- a/Dissertation.org +++ b/Dissertation.org @@ -18,8 +18,6 @@ Las nuevas técnicas de secuenciación de ADN (NGS) han revolucionado la investi *Palabras clave:* deep learning, corrección de errores, receptor de linfocitos T, secuenciación de ADN, inmunología -\clearpage - * Abstract Next generation sequencing (NGS) have revolutionised genomic research. These technologies perform sequencing of millions of fragments of DNA in parallel, which are pieced together using bioinformatics analyses. Although these techniques are commonly applied, they have non-negligible error rates that are detrimental to the analysis of regions with a high degree of polimorphism. In this study we propose a novel computational method, locimend, based on a /Deep Learning/ algorithm for DNA sequencing error correction. It is applied to the analysis of the complementarity determining region 3 (CDR3) of the T-cell receptor (TCR), generated in silico and subsequently subjected to a sequencing simulator in order to produce sequencing errors. Using these data, we trained a convolutional neural network (CNN) with the aim of generating a computational model that allows the detection and correction of sequencing errors. @@ -29,28 +27,28 @@ Next generation sequencing (NGS) have revolutionised genomic research. These tec *Keywords:* deep learning, error correction, DNA sequencing, T-cell receptor, immunology -\clearpage - * Introducción -La secuenciación de ADN es el proceso mediante el cual se determina el orden de los nucleótidos en una secuencia de ADN. En los años 70, Sanger \etal desarrollaron métodos para secuenciar el ADN mediante técnicas de terminación de cadena. cite:Sanger5463 Este avance revolucionó la biología, proporcionando las herramientas necesarias para descifrar genes, y posteriormente, genomas completos. +La secuenciación de ADN es el proceso mediante el cual se determina el orden de los nucleótidos en una secuencia de ADN. En los años 70, Sanger \etal desarrollaron métodos para secuenciar el ADN mediante técnicas de terminación de cadena. cite:Sanger5463 Este avance revolucionó la biología, proporcionando las herramientas necesarias para descifrar genes, y posteriormente, genomas completos. La demanda creciente de un mayor rendimiento llevó a la automatización y paralelización de las tareas de secuenciación. Gracias a estos avances, la técnica de Sanger permitió determinar la primera secuencia del genoma humano en 2004 (Proyecto Genoma Humano). cite:InternationalHumanGenomeSequencingConsortium2004 -La demanda creciente de un mayor rendimiento llevó a una automatización y paralelización de las tareas de secuenciación. +Sin embargo, el Proyecto Genoma Gumano requerió una gran cantidad de tiempo y recursos, y era evidente que se necesitaban tecnologías más rápidas, de mayor rendimiento y más baratas. Por esta razón, en el mismo año (2004) el /National Human Genome Research Institute/ (NHGRI) puso en marcha un programa de financiación con el objetivo de reducir el coste de la secuenciación del genoma humano a 1000 dólares en diez años. cite:Schloss2008 Esto estimuló el desarrollo y la comercialización de las tecnologías de secuenciación de alto rendimiento o /Next-Generation Sequencing/ (NGS), en contraposición con el método automatizado de Sanger, que se considera una tecnología de primera generación. +Estos nuevos métodos de secuenciación proporcionan tres mejoras importantes: en primer lugar, en lugar de requerir la clonación bacteriana de los fragmentos de ADN, se basan en la preparación de bibliotecas de moléculas en un sistema sin células. En segundo lugar, en lugar de cientos, se producen en paralelo de miles a muchos millones de reacciones de secuenciación. Finalmente, estos resultados de secuenciación se detectan directamente sin necesidad de electroforesis. cite:vanDijk2014 + +Actualmente, se encuentran en desarrollo las tecnologías de tercera generación de secuenciación (Third-Generation Sequencing). Existe un debate considerable sobre la diferencia entre la segunda y tercera generación de secuenciación, la secuenciación en tiempo real y la divergencia simple con respecto a las tecnologías anteriores deberían ser las características definitorias de la tercera generación. Aquí consideramos que las tecnologías de tercera generación son aquellas capaces de secuenciar moléculas individuales, negando el requisito de amplificación del ADN que comparten todas las tecnologías anteriores. cite:HEATHER20161 + +# Not in the correct place La capacidad del sistema inmunitario adaptativo para responder a cualquiera de los numerosos antígenos extraños potenciales a los que puede estar expuesta una persona depende de los receptores altamente polimórficos expresados por las células B (inmunoglobulinas) y las células T (receptores de células T [TCR]). La especificidad de las células T viene determinada principalmente por la secuencia de aminoácidos codificada en los bucles de la tercera región determinante de la complementariedad (CDR3). cite:pmid19706884 -** Estado del arte -*** NGS -*** Bioinformática (Deep Learning) -* Motivación y Objetivos -* Metodología +* Estado del arte +** Bioinformática +** Deep Learning +* Objetivos +* Métodos ** Tecnologías ** Pipeline ** Reproducibilidad * Resultados * Conclusiones * Futuras mejoras - -\clearpage - * Bibliografía diff --git a/Dissertation.pdf b/Dissertation.pdf index 06dab129c2a59aa202123c4fe4550698dae7efa9..a29d43be94c8f13e80c9a8a2bd5934dd8f8f3278 100644 GIT binary patch delta 47937 zcmZsCQ*|i`Lvei3l{xZUCeW1Swlo5+{`@Rt2C_4hkp&r?l#OBc87k=zE%d z&^>#3*w^OHoF8D8_SxK{Exi@QHwh_I)Pmj)ydMmw595kpvX8Pi0_KAgR>u`6;OKXT zp<8S`lWJ$=_; zR;4|?`VK!^_sLb_7CH)!`J+VKfMCm7Bqo(+kohAdsIMzetdjW_XAI02&WSa!MCu7o 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