From d17f0bc2d5f7afe21ea2c235ea9e3058e1d02387 Mon Sep 17 00:00:00 2001 From: coolneng Date: Sun, 27 Jun 2021 02:55:31 +0200 Subject: [PATCH] Add English abstract --- docs/Dissertation.org | 5 ++++- docs/Dissertation.pdf | Bin 419208 -> 420494 bytes 2 files changed, 4 insertions(+), 1 deletion(-) diff --git a/docs/Dissertation.org b/docs/Dissertation.org index f10e457..9c24fff 100644 --- a/docs/Dissertation.org +++ b/docs/Dissertation.org @@ -12,10 +12,13 @@ #+PANDOC_METADATA: logo:/home/coolneng/Photos/Logos/UGR.png * Resumen -Las nuevas técnicas de secuenciación de ADN (NGS) han revolucionado la investigación en genómica. Estas tecnologías se basan en la secuenciación de millones de fragmentos de ADN en paralelo, cuya reconstrucción se basa en técnicas de bioinformática. Aunque estas técnicas se apliquen de forma habitual, presentan tasas de error significantes que son detrimentales para el análisis de regiones con alto grado de polimorfismo. En este estudio se implementa un nuevo método computacional, locimend, basado en /Deep Learning/ para la corrección de errores de secuenciación. Se aplica al análisis de la región determinante de complementariedad 3 (CDR3) del receptor de linfocitos T (TCR), generada /in silico/ y posteriorimente sometida a un simulador de secuenciación con el fin de producir errores de secuenciación. Empleando estos datos, entrenamos una red neuronal convolucional (CNN) con el objetivo de generar un modelo computacional que permita la detección y corrección de los errores de secuenciación. +Las nuevas técnicas de secuenciación de ADN (NGS) han revolucionado la investigación en genómica. Estas tecnologías se basan en la secuenciación de millones de fragmentos de ADN en paralelo, cuya reconstrucción se basa en técnicas de bioinformática. Aunque estas técnicas se apliquen de forma habitual, presentan tasas de error significantes que son detrimentales para el análisis de regiones con alto grado de polimorfismo. En este estudio se implementa un nuevo método computacional, locimend, basado en /Deep Learning/ para la corrección de errores de secuenciación de ADN. Se aplica al análisis de la región determinante de complementariedad 3 (CDR3) del receptor de linfocitos T (TCR), generada /in silico/ y posteriorimente sometida a un simulador de secuenciación con el fin de producir errores de secuenciación. Empleando estos datos, entrenamos una red neuronal convolucional (CNN) con el objetivo de generar un modelo computacional que permita la detección y corrección de los errores de secuenciación. # TODO Add results * Abstract + +Next generation sequencing (NGS) have revolutionised genomic research. These technologies perform sequencing of millions of fragments of DNA in parallel, which are pieced together using bioinformatics analyses. Although these techniques are commonly applied, they have non-negligible error rates that are detrimental to the analysis of regions with a high degree of polimorphism. In this study we propose a novel computational method, locimend, based on a /Deep Learning/ algorithm for DNA sequencing error correction. It is applied to the analysis of the complementarity determining region 3 (CDR3) of the T-cell receptor (TCR), generated in silico and subsequently subjected to a sequencing simulator in order to produce sequencing errors. Using these data, we trained a convolutional neural network (CNN) with the aim of generating a computational model that allows the detection and correction of sequencing errors. + * Introducción * Motivación * Estado del arte diff --git a/docs/Dissertation.pdf b/docs/Dissertation.pdf index 8beeb8ae9c65b7545bc67099c0e034f5a2b1d029..fae468401830076956c59d56734fd79cbc7e0623 100644 GIT binary patch delta 34534 zcmV(`K-0g7%NdT<8GwWVgaWh!qt6mz-!Px*g*z^J+tV>R{}mYd4!5<>0~P@U72eL} zm(S1xCIvDvGcX{Rfz|{kf1OxMbK^D=zWZ13NLJYhK=65-WG1O(XI-^3+EbhZEzuGZ z64Z#|&idb{8{MEtSjy`n5@@i2{`&PnGTKKn`r+=|&D}TOXL+QgsAa99%}Z2DQ58j0 z=9N%+8f|vb&+)&T?ZMbKc}S&?tDa;Ly}8L5k{T2xgPZTq{Qf05A+WIshx=(>u&x|MyD!IC`F@ZLqA@BVWq z8Nm4oU=>vnh0oJIf4U|5vl?j~{=@R}l6_v~h{!awW)B`MNL%4b9LU^mQwTVSl z30-E2LRf5(=edyJ0OqNL{&3xp|B zLI#d5%2X(o6AB+jH2aMD(L`0(6lm?%4&AT^2y#C^w)A{OASKPXp`oTvT~M z*3W4Psb>j^f729XsZ!4J6jpl6Y1Ton5Im*d#){%JgoV#$F!qJ=yMNM|a_KyK$*p;p z6!B!gOuQ2Sr0R=X{&1U-w`q=@0R?usxr|?u8n?X(hpt6lmjuV5c{b;%=>W+z&Eq3i 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